Nanoplasmonic sensing and capillary electrophoresis for fast screening of interactions between phosphatidylcholine biomembranes and surfactants

Filip Dusa, Wen Chen, Joanna Magdalena Witos, Susanne Kristina Wiedmer

Forskningsoutput: TidskriftsbidragArtikelVetenskapligPeer review

Sammanfattning

Nanoplasmonic sensing (NPS), based on localized surface plasmon resonance, with sensors composed of glass covered with golden nanodisks and overlaid with a SiO2 coating was applied in this study. Egg phosphatidylcholine (eggPC), being an easily accessible membrane-forming lipid, was used for preparation of biomimicking membranes. Small unilamellar vesicles with an approximate hydrodynamic diameter of 30 nm, formed by sonication in HEPES buffer, were
adsorbed within 10 min on the sensor surface either as intact vesicles or as a planar bilayer. The adsorbed biomembrane systems were further utilized for interaction studies with four different well-known surfactants (negatively and positively charged, zwitterionic, and non-ionic) and each surfactant was tested at concentrations below and above the critical micelle concentration (CMC).
Our results allowed the evaluation of different NPS patterns for every particular supported membrane system, surfactant, and its concentration. The most significant effect on the membrane was achieved upon the introduction of zwitterionic surfactant micelles, which in fact completely solubilized and removed the lipid membranes from the sensor surface. Other surfactant micelles
interacted with the membranes and formed mixed structures remaining on the sensor surface. The studies performed at the concentrations below the CMCs of the surfactants showed that different mixed systems were formed. Depending on the supported membrane system and the type of surfactant, the mixed systems indicated different formation kinetics. Additionally, the final water
rinse revealed the stability of the formed systems. To investigate the effect of the studied surfactants on the overall surface charge of the biomembrane, capillary electrophoresis (CE) experiments were carried out in parallel with the NPS analysis. The electroosmotic flow mobility of an eggPC-coated fused silica capillary was used to measure the total surface charge of the biomembrane after its treatment with the surfactants. Our results indicated in general good correlation between CE and NPS data. However, some discrepancies were seen while applying either zwitterionic or positively charged surfactants. This confirmed that CE analysis was able to provide additional data about the investigated systems. Taken together, the combination of NPS and CE proved to be an efficient way to describe the nature of interactions between biomimicking
membranes and amphiphilic molecules.
Originalspråkengelska
TidskriftLangmuir
Volym34
Utgåva20
Sidor (från-till)5889-5900
Antal sidor12
ISSN0743-7463
DOI
StatusPublicerad - 22 maj 2018
MoE-publikationstypA1 Tidskriftsartikel-refererad

Vetenskapsgrenar

  • 116 Kemi

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title = "Nanoplasmonic sensing and capillary electrophoresis for fast screening of interactions between phosphatidylcholine biomembranes and surfactants",
abstract = "Nanoplasmonic sensing (NPS), based on localized surface plasmon resonance, with sensors composed of glass covered with golden nanodisks and overlaid with a SiO2 coating was applied in this study. Egg phosphatidylcholine (eggPC), being an easily accessible membrane-forming lipid, was used for preparation of biomimicking membranes. Small unilamellar vesicles with an approximate hydrodynamic diameter of 30 nm, formed by sonication in HEPES buffer, wereadsorbed within 10 min on the sensor surface either as intact vesicles or as a planar bilayer. The adsorbed biomembrane systems were further utilized for interaction studies with four different well-known surfactants (negatively and positively charged, zwitterionic, and non-ionic) and each surfactant was tested at concentrations below and above the critical micelle concentration (CMC).Our results allowed the evaluation of different NPS patterns for every particular supported membrane system, surfactant, and its concentration. The most significant effect on the membrane was achieved upon the introduction of zwitterionic surfactant micelles, which in fact completely solubilized and removed the lipid membranes from the sensor surface. Other surfactant micellesinteracted with the membranes and formed mixed structures remaining on the sensor surface. The studies performed at the concentrations below the CMCs of the surfactants showed that different mixed systems were formed. Depending on the supported membrane system and the type of surfactant, the mixed systems indicated different formation kinetics. Additionally, the final waterrinse revealed the stability of the formed systems. To investigate the effect of the studied surfactants on the overall surface charge of the biomembrane, capillary electrophoresis (CE) experiments were carried out in parallel with the NPS analysis. The electroosmotic flow mobility of an eggPC-coated fused silica capillary was used to measure the total surface charge of the biomembrane after its treatment with the surfactants. Our results indicated in general good correlation between CE and NPS data. However, some discrepancies were seen while applying either zwitterionic or positively charged surfactants. This confirmed that CE analysis was able to provide additional data about the investigated systems. Taken together, the combination of NPS and CE proved to be an efficient way to describe the nature of interactions between biomimickingmembranes and amphiphilic molecules.",
keywords = "116 Chemical sciences, LIPID-BILAYER FORMATION, SODIUM DODECYL-SULFATE, DETERGENT SOLUBILIZATION, NANOMATERIALS SCIENCE, COATED CAPILLARIES, MIXED MICELLES, IONIC LIQUIDS, TRITON X-100, MEMBRANE, VESICLES",
author = "Filip Dusa and Wen Chen and Witos, {Joanna Magdalena} and Wiedmer, {Susanne Kristina}",
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month = "5",
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journal = "Langmuir",
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publisher = "American Chemical Society",
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Nanoplasmonic sensing and capillary electrophoresis for fast screening of interactions between phosphatidylcholine biomembranes and surfactants. / Dusa, Filip; Chen, Wen; Witos, Joanna Magdalena; Wiedmer, Susanne Kristina.

I: Langmuir, Vol. 34, Nr. 20, 22.05.2018, s. 5889-5900.

Forskningsoutput: TidskriftsbidragArtikelVetenskapligPeer review

TY - JOUR

T1 - Nanoplasmonic sensing and capillary electrophoresis for fast screening of interactions between phosphatidylcholine biomembranes and surfactants

AU - Dusa, Filip

AU - Chen, Wen

AU - Witos, Joanna Magdalena

AU - Wiedmer, Susanne Kristina

PY - 2018/5/22

Y1 - 2018/5/22

N2 - Nanoplasmonic sensing (NPS), based on localized surface plasmon resonance, with sensors composed of glass covered with golden nanodisks and overlaid with a SiO2 coating was applied in this study. Egg phosphatidylcholine (eggPC), being an easily accessible membrane-forming lipid, was used for preparation of biomimicking membranes. Small unilamellar vesicles with an approximate hydrodynamic diameter of 30 nm, formed by sonication in HEPES buffer, wereadsorbed within 10 min on the sensor surface either as intact vesicles or as a planar bilayer. The adsorbed biomembrane systems were further utilized for interaction studies with four different well-known surfactants (negatively and positively charged, zwitterionic, and non-ionic) and each surfactant was tested at concentrations below and above the critical micelle concentration (CMC).Our results allowed the evaluation of different NPS patterns for every particular supported membrane system, surfactant, and its concentration. The most significant effect on the membrane was achieved upon the introduction of zwitterionic surfactant micelles, which in fact completely solubilized and removed the lipid membranes from the sensor surface. Other surfactant micellesinteracted with the membranes and formed mixed structures remaining on the sensor surface. The studies performed at the concentrations below the CMCs of the surfactants showed that different mixed systems were formed. Depending on the supported membrane system and the type of surfactant, the mixed systems indicated different formation kinetics. Additionally, the final waterrinse revealed the stability of the formed systems. To investigate the effect of the studied surfactants on the overall surface charge of the biomembrane, capillary electrophoresis (CE) experiments were carried out in parallel with the NPS analysis. The electroosmotic flow mobility of an eggPC-coated fused silica capillary was used to measure the total surface charge of the biomembrane after its treatment with the surfactants. Our results indicated in general good correlation between CE and NPS data. However, some discrepancies were seen while applying either zwitterionic or positively charged surfactants. This confirmed that CE analysis was able to provide additional data about the investigated systems. Taken together, the combination of NPS and CE proved to be an efficient way to describe the nature of interactions between biomimickingmembranes and amphiphilic molecules.

AB - Nanoplasmonic sensing (NPS), based on localized surface plasmon resonance, with sensors composed of glass covered with golden nanodisks and overlaid with a SiO2 coating was applied in this study. Egg phosphatidylcholine (eggPC), being an easily accessible membrane-forming lipid, was used for preparation of biomimicking membranes. Small unilamellar vesicles with an approximate hydrodynamic diameter of 30 nm, formed by sonication in HEPES buffer, wereadsorbed within 10 min on the sensor surface either as intact vesicles or as a planar bilayer. The adsorbed biomembrane systems were further utilized for interaction studies with four different well-known surfactants (negatively and positively charged, zwitterionic, and non-ionic) and each surfactant was tested at concentrations below and above the critical micelle concentration (CMC).Our results allowed the evaluation of different NPS patterns for every particular supported membrane system, surfactant, and its concentration. The most significant effect on the membrane was achieved upon the introduction of zwitterionic surfactant micelles, which in fact completely solubilized and removed the lipid membranes from the sensor surface. Other surfactant micellesinteracted with the membranes and formed mixed structures remaining on the sensor surface. The studies performed at the concentrations below the CMCs of the surfactants showed that different mixed systems were formed. Depending on the supported membrane system and the type of surfactant, the mixed systems indicated different formation kinetics. Additionally, the final waterrinse revealed the stability of the formed systems. To investigate the effect of the studied surfactants on the overall surface charge of the biomembrane, capillary electrophoresis (CE) experiments were carried out in parallel with the NPS analysis. The electroosmotic flow mobility of an eggPC-coated fused silica capillary was used to measure the total surface charge of the biomembrane after its treatment with the surfactants. Our results indicated in general good correlation between CE and NPS data. However, some discrepancies were seen while applying either zwitterionic or positively charged surfactants. This confirmed that CE analysis was able to provide additional data about the investigated systems. Taken together, the combination of NPS and CE proved to be an efficient way to describe the nature of interactions between biomimickingmembranes and amphiphilic molecules.

KW - 116 Chemical sciences

KW - LIPID-BILAYER FORMATION

KW - SODIUM DODECYL-SULFATE

KW - DETERGENT SOLUBILIZATION

KW - NANOMATERIALS SCIENCE

KW - COATED CAPILLARIES

KW - MIXED MICELLES

KW - IONIC LIQUIDS

KW - TRITON X-100

KW - MEMBRANE

KW - VESICLES

U2 - 10.1021/acs.langmuir.8b01074

DO - 10.1021/acs.langmuir.8b01074

M3 - Article

VL - 34

SP - 5889

EP - 5900

JO - Langmuir

JF - Langmuir

SN - 0743-7463

IS - 20

ER -