Neurotrophic factors switch between two signaling pathways that trigger axonal growth

Mikhail Paveliev, Maria Lume, Agne Velthut, Matthew Phillips, Urmas Arumäe, Mart Saarma

    Forskningsoutput: TidskriftsbidragArtikelVetenskapligPeer review

    Sammanfattning

    Integration of multiple inputs from the extracellular environment, such as extracellular matrix molecules and growth factors, is a crucial process for cell function and information processing in multicellular organisms. Here we demonstrate that co-stimulation of dorsal root ganglion neurons with neurotrophic factors ( NTFs)-glial-cell-line-derived neurotrophic factor, neurturin or nerve growth factor-and laminin leads to axonal growth that requires activation of Src family kinases ( SFKs). A different, SFK-independent signaling pathway evokes axonal growth on laminin in the absence of the NTFs. By contrast, axonal branching is regulated by SFKs both in the presence and in the absence of NGF. We propose and experimentally verify a Boolean model of the signaling network triggered by NTFs and laminin. Our results demonstrate that NTFs provide an environmental cue that triggers a switch between separate pathways in the cell signaling network.
    Originalspråkengelska
    TidskriftJournal of Cell Science
    Volym120
    Sidor (från-till)2507-2516
    Antal sidor10
    ISSN0021-9533
    DOI
    StatusPublicerad - 2007
    MoE-publikationstypA1 Tidskriftsartikel-refererad

    Citera det här

    Paveliev, Mikhail ; Lume, Maria ; Velthut, Agne ; Phillips, Matthew ; Arumäe, Urmas ; Saarma, Mart. / Neurotrophic factors switch between two signaling pathways that trigger axonal growth. I: Journal of Cell Science. 2007 ; Vol. 120. s. 2507-2516.
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    abstract = "Integration of multiple inputs from the extracellular environment, such as extracellular matrix molecules and growth factors, is a crucial process for cell function and information processing in multicellular organisms. Here we demonstrate that co-stimulation of dorsal root ganglion neurons with neurotrophic factors ( NTFs)-glial-cell-line-derived neurotrophic factor, neurturin or nerve growth factor-and laminin leads to axonal growth that requires activation of Src family kinases ( SFKs). A different, SFK-independent signaling pathway evokes axonal growth on laminin in the absence of the NTFs. By contrast, axonal branching is regulated by SFKs both in the presence and in the absence of NGF. We propose and experimentally verify a Boolean model of the signaling network triggered by NTFs and laminin. Our results demonstrate that NTFs provide an environmental cue that triggers a switch between separate pathways in the cell signaling network.",
    author = "Mikhail Paveliev and Maria Lume and Agne Velthut and Matthew Phillips and Urmas Arum{\"a}e and Mart Saarma",
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    Neurotrophic factors switch between two signaling pathways that trigger axonal growth. / Paveliev, Mikhail; Lume, Maria; Velthut, Agne; Phillips, Matthew; Arumäe, Urmas; Saarma, Mart.

    I: Journal of Cell Science, Vol. 120, 2007, s. 2507-2516.

    Forskningsoutput: TidskriftsbidragArtikelVetenskapligPeer review

    TY - JOUR

    T1 - Neurotrophic factors switch between two signaling pathways that trigger axonal growth

    AU - Paveliev, Mikhail

    AU - Lume, Maria

    AU - Velthut, Agne

    AU - Phillips, Matthew

    AU - Arumäe, Urmas

    AU - Saarma, Mart

    PY - 2007

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    AB - Integration of multiple inputs from the extracellular environment, such as extracellular matrix molecules and growth factors, is a crucial process for cell function and information processing in multicellular organisms. Here we demonstrate that co-stimulation of dorsal root ganglion neurons with neurotrophic factors ( NTFs)-glial-cell-line-derived neurotrophic factor, neurturin or nerve growth factor-and laminin leads to axonal growth that requires activation of Src family kinases ( SFKs). A different, SFK-independent signaling pathway evokes axonal growth on laminin in the absence of the NTFs. By contrast, axonal branching is regulated by SFKs both in the presence and in the absence of NGF. We propose and experimentally verify a Boolean model of the signaling network triggered by NTFs and laminin. Our results demonstrate that NTFs provide an environmental cue that triggers a switch between separate pathways in the cell signaling network.

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    DO - 10.1242/jcs.003590

    M3 - Article

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    JO - Journal of Cell Science

    JF - Journal of Cell Science

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