Patient-Specific Bioimplants and Reconstruction Plates for Mandibular Defects: Production Workflow and In Vivo Large Animal Model Study

Kasper Dienel, Ahmed Abu-Shahba, Roman Kornilov, Roy Björkstrand, Bas van Bochove, Johanna Snäll, Tommy S E Wilkman, Karri Mesimäki, Anna Meller, Jere Linden, Anu Lappalainen, Jouni Partanen, Riitta Seppänen-Kaijansinkko, Jukka Seppälä, Bettina Mannerström

Forskningsoutput: TidskriftsbidragArtikelVetenskapligPeer review


A major challenge with extensive craniomaxillofacial bone reconstruction is the limited donor-site availability to reconstruct defects predictably and accurately according to the anatomical shape of the patient. Here, patient-specific composite bioimplants, consisting of cross-linked poly(trimethylene carbonate) (PTMC) networks and beta-tricalcium phosphate (beta-TCP), are tested in vivo in twelve Gottingen minipigs in a large mandibular continuity defect model. The 25 mm defects are supported by patient-specific titanium reconstruction plates and receive either osteoconductive composite bioimplants (PTMC+TCP), neat polymer network bioimplants (PTMC), autologous bone segments (positive control), or are left empty (negative control). Postoperatively, defects treated with bioimplants show evident ossification at 24 weeks. Histopathologic evaluation reveals that neat PTMC bioimplant surfaces are largely covered with fibrous tissue, while in the PTMC+TCP bioimplants, bone attached directly to the implant surface shows good osteoconduction and histological signs of osteoinductivity. However, PTMC+TCP bioimplants are associated with high incidence of necrosis and infection, possibly due to rapid resorption and/or particle size of the used beta-TCP. The study highlights the importance of testing bone regeneration implants in a clinically relevant large animal model and at the in situ reconstruction site, since results on small animal models and studies in nonloadbearing areas do not translate directly.

TidskriftMacromolecular Bioscience
Antal sidor15
StatusPublicerad - apr. 2022
MoE-publikationstypA1 Tidskriftsartikel-refererad


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