Patients with post-COVID-19 condition show minor blood transcriptomic changes, with altered erythrocyte gene expression in a male subgroup

Piia Karisola, Mari Kanerva, Aki Vuokko, Helena Liira, Shuyuan Wang, Kirsi Kvarnström, Mikko Varonen, Hille Suojalehto, Harri Alenius

Forskningsoutput: TidskriftsbidragArtikelVetenskapligPeer review

Sammanfattning

Background: The mechanisms underlying persistent symptoms after non-severe COVID-19 remain unclear. This study aimed to investigate transcriptomic changes in peripheral blood cells of patients with post-COVID-19 condition (PCC) and assess if distinct clinical subtypes with specific gene signatures could be identified. Methods: The cohort included 111 PCC patients from the SARS-CoV-2 Omicron variant era, with 57 recovered (Recov) and 54 having prolonged symptoms indicative of PCC. The results were compared to 63 healthy controls (Ctrl) without known SARS-CoV-2 infection. Clinical data included patient assessments, laboratory results, comorbidities, and questionnaires on quality of life and functioning. Transcriptomic analysis and cellular deconvolution methods were used on total RNA from peripheral blood mononuclear cells (PBMCs). Results: PCC patients had more comorbidities (mean 1.3) and more frequently (59%) at least one comorbidity than recovered patients (31%) and controls (24%). Overall, past COVID-19 illness or current PCC symptoms caused minimal changes in the blood cell transcriptome, with only 3–6 differentially expressed genes (DEGs) identified across comparisons. However, a subset of male PCC patients exhibited an increased fraction of deconvoluted erythroblasts and significant genome-wide gene expression changes, with 399 DEGs compared to recovered and control males. These genes were enriched in pathways related to heme metabolism and gas exchange in erythrocytes. Conclusions: Persistent symptoms in PCC are multifactorial and not directly linked to peripheral blood cell gene expression changes. However, a subgroup of male PCC patients shows distinct erythrocyte responses that may contribute to long-term symptoms.

Originalspråkengelska
Artikelnummer1500997
TidskriftFrontiers in Immunology
Volym16
Antal sidor13
ISSN1664-3224
DOI
StatusPublicerad - 21 mars 2025
MoE-publikationstypA1 Tidskriftsartikel-refererad

Bibliografisk information

Publisher Copyright:
Copyright © 2025 Karisola, Kanerva, Vuokko, Liira, Wang, Kvarnström, Varonen, Suojalehto and Alenius.

Vetenskapsgrenar

  • 3121 Allmänmedicin, inre medicin och annan klinisk medicin

Citera det här