@inbook{ad76e4ebc5ad49d9b320b6c3f33a5a12,
title = "Studying Golgi Structure and Function by Thin Section TEM",
abstract = "Here, we describe protocols for chemical fixation and flat embedding to study the Golgi structure by thin section transmission electron microscopy (TEM) and for 3,3'-diaminobenzidine (DAB) cytochemical staining and pre-embedding immunolabelling to localize specific Golgi proteins. Furthermore, we demonstrate how the Golgi morphology can be elucidated by classifying the Golgi membranes using Microscopy Image Browser-a software that provides anonymization, modelling, and annotation.",
keywords = "1182 Biochemistry, cell and molecular biology",
author = "Helena Vihinen and Eija Jokitalo",
year = "2023",
doi = "10.1007/978-1-0716-2639-9_11",
language = "English",
isbn = "978-1-0716-2638-2",
series = "Methods in molecular biology",
publisher = "Humana press",
pages = "141--159",
editor = "Yanzhuang Wang and Lupashin, {Vladimir V.} and Graham, {Todd R.}",
booktitle = "Golgi",
address = "United States",
}