Temporal transcriptome analysis of the white-rot fungus Obba rivulosa shows expression of a constitutive set of plant cell wall degradation targeted genes during growth on solid spruce wood

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The basidiomycete white-rot fungus Obba rivulosa, a close relative of Gelatoporia (Ceriporiopsis) subvermispora, is an efficient degrader of softwood. The dikaryotic O. rivulosa strain T241i (FBCC949) has been shown to selectively remove lignin from spruce wood prior to depolymerization of plant cell wall polysaccharides, thus possessing potential in biotechnological applications such as pretreatment of wood in pulp and paper industry. In this work, we studied the time-course of the conversion of spruce by the genome-sequenced monokaryotic O. rivulosa strain 3A-2, which is derived from the dikaryon T241i, to get insight into transcriptome level changes during prolonged solid state cultivation. During 8-week cultivation, O. rivulosa expressed a constitutive set of genes encoding putative plant cell wall degrading enzymes. High level of expression of the genes targeted towards all plant cell wall polymers was detected at 2-week time point, after which majority of the genes showed reduced expression. This implicated non-selective degradation of lignin by the O. rivulosa monokaryon and suggests high variation between mono- and dikaryotic strains of the white-rot fungi with respect to their abilities to convert plant cell wall polymers.
Originalspråkengelska
TidskriftFungal Genetics and Biology
Volym112
Sidor (från-till)47-54
Antal sidor8
ISSN1087-1845
DOI
StatusPublicerad - mar 2018
MoE-publikationstypA1 Tidskriftsartikel-refererad

Vetenskapsgrenar

  • 1184 Genetik, utvecklingsbiologi, fysiologi

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@article{1c0c69b7dd284bdda4c43c4945053953,
title = "Temporal transcriptome analysis of the white-rot fungus Obba rivulosa shows expression of a constitutive set of plant cell wall degradation targeted genes during growth on solid spruce wood",
abstract = "The basidiomycete white-rot fungus Obba rivulosa, a close relative of Gelatoporia (Ceriporiopsis) subvermispora, is an efficient degrader of softwood. The dikaryotic O. rivulosa strain T241i (FBCC949) has been shown to selectively remove lignin from spruce wood prior to depolymerization of plant cell wall polysaccharides, thus possessing potential in biotechnological applications such as pretreatment of wood in pulp and paper industry. In this work, we studied the time-course of the conversion of spruce by the genome-sequenced monokaryotic O. rivulosa strain 3A-2, which is derived from the dikaryon T241i, to get insight into transcriptome level changes during prolonged solid state cultivation. During 8-week cultivation, O. rivulosa expressed a constitutive set of genes encoding putative plant cell wall degrading enzymes. High level of expression of the genes targeted towards all plant cell wall polymers was detected at 2-week time point, after which majority of the genes showed reduced expression. This implicated non-selective degradation of lignin by the O. rivulosa monokaryon and suggests high variation between mono- and dikaryotic strains of the white-rot fungi with respect to their abilities to convert plant cell wall polymers.",
keywords = "1184 Genetics, developmental biology, physiology, Obba rivulosa, White-rot, Lignocellulose, Solid state cultivation, Transcriptomics, CERIPORIOPSIS-SUBVERMISPORA, PHANEROCHAETE-CHRYSOSPORIUM, PHYSISPORINUS-RIVULOSUS, OXALIC-ACID, MANGANESE PEROXIDASES, OXALATE DECARBOXYLASE, DICHOMITUS-SQUALENS, SECRETOME ANALYSIS, POSTIA-PLACENTA, ENZYMES",
author = "Mila Marinovic and Aguilar-Pontes, {Maria Victoria} and Miaomiao Zhou and Miettinen, {Otto Kullervo} and {de Vries}, Ronald and M{\"a}kel{\"a}, {Miia Riitta} and Hilden, {Sari Kristiina}",
year = "2018",
month = "3",
doi = "10.1016/j.fgb.2017.07.004",
language = "English",
volume = "112",
pages = "47--54",
journal = "Fungal Genetics and Biology",
issn = "1087-1845",
publisher = "ACADEMIC PRESS INC ELSEVIER SCIENCE",

}

TY - JOUR

T1 - Temporal transcriptome analysis of the white-rot fungus Obba rivulosa shows expression of a constitutive set of plant cell wall degradation targeted genes during growth on solid spruce wood

AU - Marinovic, Mila

AU - Aguilar-Pontes, Maria Victoria

AU - Zhou, Miaomiao

AU - Miettinen, Otto Kullervo

AU - de Vries, Ronald

AU - Mäkelä, Miia Riitta

AU - Hilden, Sari Kristiina

PY - 2018/3

Y1 - 2018/3

N2 - The basidiomycete white-rot fungus Obba rivulosa, a close relative of Gelatoporia (Ceriporiopsis) subvermispora, is an efficient degrader of softwood. The dikaryotic O. rivulosa strain T241i (FBCC949) has been shown to selectively remove lignin from spruce wood prior to depolymerization of plant cell wall polysaccharides, thus possessing potential in biotechnological applications such as pretreatment of wood in pulp and paper industry. In this work, we studied the time-course of the conversion of spruce by the genome-sequenced monokaryotic O. rivulosa strain 3A-2, which is derived from the dikaryon T241i, to get insight into transcriptome level changes during prolonged solid state cultivation. During 8-week cultivation, O. rivulosa expressed a constitutive set of genes encoding putative plant cell wall degrading enzymes. High level of expression of the genes targeted towards all plant cell wall polymers was detected at 2-week time point, after which majority of the genes showed reduced expression. This implicated non-selective degradation of lignin by the O. rivulosa monokaryon and suggests high variation between mono- and dikaryotic strains of the white-rot fungi with respect to their abilities to convert plant cell wall polymers.

AB - The basidiomycete white-rot fungus Obba rivulosa, a close relative of Gelatoporia (Ceriporiopsis) subvermispora, is an efficient degrader of softwood. The dikaryotic O. rivulosa strain T241i (FBCC949) has been shown to selectively remove lignin from spruce wood prior to depolymerization of plant cell wall polysaccharides, thus possessing potential in biotechnological applications such as pretreatment of wood in pulp and paper industry. In this work, we studied the time-course of the conversion of spruce by the genome-sequenced monokaryotic O. rivulosa strain 3A-2, which is derived from the dikaryon T241i, to get insight into transcriptome level changes during prolonged solid state cultivation. During 8-week cultivation, O. rivulosa expressed a constitutive set of genes encoding putative plant cell wall degrading enzymes. High level of expression of the genes targeted towards all plant cell wall polymers was detected at 2-week time point, after which majority of the genes showed reduced expression. This implicated non-selective degradation of lignin by the O. rivulosa monokaryon and suggests high variation between mono- and dikaryotic strains of the white-rot fungi with respect to their abilities to convert plant cell wall polymers.

KW - 1184 Genetics, developmental biology, physiology

KW - Obba rivulosa

KW - White-rot

KW - Lignocellulose

KW - Solid state cultivation

KW - Transcriptomics

KW - CERIPORIOPSIS-SUBVERMISPORA

KW - PHANEROCHAETE-CHRYSOSPORIUM

KW - PHYSISPORINUS-RIVULOSUS

KW - OXALIC-ACID

KW - MANGANESE PEROXIDASES

KW - OXALATE DECARBOXYLASE

KW - DICHOMITUS-SQUALENS

KW - SECRETOME ANALYSIS

KW - POSTIA-PLACENTA

KW - ENZYMES

U2 - 10.1016/j.fgb.2017.07.004

DO - 10.1016/j.fgb.2017.07.004

M3 - Article

VL - 112

SP - 47

EP - 54

JO - Fungal Genetics and Biology

JF - Fungal Genetics and Biology

SN - 1087-1845

ER -