The Cre/Lox System to Assess the Development of the Mouse Brain

Claudius Frank Kratochwil, Filippo M. Rijli

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Sammanfattning

Cre-mediated recombination has become a powerful tool to confine gene deletions (conditional knock- outs) or overexpression of genes (conditional knockin/overexpression). By spatiotemporal restriction of genetic manipulations, major problems of classical knockouts such as embryonic lethality can be circum- vented. Furthermore Cre-mediated recombination has broad applicability in the analysis of the cellular behavior of subpopulations and cell types as well as for genetic fate mapping. This chapter will give an overview about applications for the Cre/LoxP system and their execution.
Originalspråkengelska
Titel på gästpublikationBrain Development: Methods and Protocols : Methods in Molecular Biology
RedaktörerSimon Sprecher
Antal sidor19
Volym1082
UtgivningsortTotowa, NJ
FörlagHumana press
Utgivningsdatum2014
Sidor295-313
ISBN (tryckt)978-1-62703-654-2
ISBN (elektroniskt)978-1-62703-655-9
StatusPublicerad - 2014
MoE-publikationstypB2 Del av bok eller annan forskningsbok

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