The Cre/Lox System to Assess the Development of the Mouse Brain

Claudius Frank Kratochwil, Filippo M. Rijli

Forskningsoutput: Kapitel i bok/rapport/konferenshandlingKapitelVetenskaplig

Sammanfattning

Cre-mediated recombination has become a powerful tool to confine gene deletions (conditional knockouts) or overexpression of genes (conditional knockin/overexpression). By spatiotemporal restriction of genetic manipulations, major problems of classical knockouts such as embryonic lethality or pleiotropy can be circumvented. Furthermore, Cre-mediated recombination has broad applications in the analysis of the cellular behavior of subpopulations and cell types as well as for genetic fate mapping. This chapter gives an overview about applications for the Cre/LoxP system and their execution.
Originalspråkengelska
Titel på gästpublikationBrain Development: Methods in Molecular Biology : Methods in Molecular Biology
RedaktörerSimon Sprecher
Volym2047
UtgivningsortNew York, NY
FörlagHumana press
Utgivningsdatum2020
ISBN (tryckt)978-1-4939-9731-2
ISBN (elektroniskt)978-1-4939-9732-9
StatusPublicerad - 2020
MoE-publikationstypB2 Del av bok eller annan forskningsbok

Citera det här