The layered fold of the TSR domain of P. falciparum TRAP contains a heparin binding site

Helena Tossavainen, Tero Pihlajamaa, Toni K Huttunen, Erkki Raulo, Heikki Rauvala, Perttu Permi, Ilkka Kilpeläinen

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    Sammanfattning

    Thrombospondin-related anonymous protein, TRAP, has a critical role in the hepatocyte invasion step of Plasmodium sporozoites, the transmissible form of the parasite causing malaria. The extracellular domains of this sporozoite surface protein interact with hepatocyte surface receptors whereas its intracellular domain acts as a link to the sporozoite actomyosin motor system. Liver heparan sulfate proteoglycans have been identified as potential ligands for TRAP. Proteoglycan binding has been associated with the A- and TSR domains of TRAP. We present the solution NMR structure of the TSR domain of TRAP and a chemical shift mapping study of its heparin binding epitope. The domain has an elongated structure stabilized by an array of tryptophan and arginine residues as well as disulfide bonds. The fold is very similar to those of thrombospondin type-1 (TSP-1) and F-spondin TSRs. The heparin binding site of TRAP-TSR is located in the N-terminal half of the structure, the layered side chains forming an integral part of the site. The smallest heparin fragment capable of binding to TRAP-TSR is a tetrasaccharide.
    Originalspråkengelska
    TidskriftProtein Science
    Volym15
    Utgåva7
    Sidor (från-till)1760-1768
    Antal sidor9
    ISSN0961-8368
    DOI
    StatusPublicerad - 2006
    MoE-publikationstypA1 Tidskriftsartikel-refererad

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