Three dimensional electron microscopy of cellular organelles by serial block face SEM and ET

Forskningsoutput: TidskriftsbidragArtikelVetenskaplig

Sammanfattning

Novel serial imaging methods using scanning electron microscopy may be used for 3D morphological studies of plastic embedded biological specimens. Serial block face SEM has already proven an undisputable position in 3D-EM studies of tissues, but it has a lot of potential in cell biology applications too. Electron tomography can be used to complement those studies where higher resolution is needed. In this article we present examples of utilizing these two 3D-EM techniques to study cellular organelles.
Originalspråkengelska
TidskriftMicroscopy and Analysis
Volym27
Utgåva2
Sidor (från-till)7-10
Antal sidor4
ISSN2049-4424
StatusPublicerad - 2013
MoE-publikationstypB1 Artikel i en vetenskaplig tidskrift

Vetenskapsgrenar

  • 1182 Biokemi, cell- och molekylärbiologi

Citera det här

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title = "Three dimensional electron microscopy of cellular organelles by serial block face SEM and ET",
abstract = "Novel serial imaging methods using scanning electron microscopy may be used for 3D morphological studies of plastic embedded biological specimens. Serial block face SEM has already proven an undisputable position in 3D-EM studies of tissues, but it has a lot of potential in cell biology applications too. Electron tomography can be used to complement those studies where higher resolution is needed. In this article we present examples of utilizing these two 3D-EM techniques to study cellular organelles.",
keywords = "1182 Biochemistry, cell and molecular biology",
author = "Helena Vihinen and Ilya Belevich and Eija Jokitalo",
year = "2013",
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AU - Vihinen, Helena

AU - Belevich, Ilya

AU - Jokitalo, Eija

PY - 2013

Y1 - 2013

N2 - Novel serial imaging methods using scanning electron microscopy may be used for 3D morphological studies of plastic embedded biological specimens. Serial block face SEM has already proven an undisputable position in 3D-EM studies of tissues, but it has a lot of potential in cell biology applications too. Electron tomography can be used to complement those studies where higher resolution is needed. In this article we present examples of utilizing these two 3D-EM techniques to study cellular organelles.

AB - Novel serial imaging methods using scanning electron microscopy may be used for 3D morphological studies of plastic embedded biological specimens. Serial block face SEM has already proven an undisputable position in 3D-EM studies of tissues, but it has a lot of potential in cell biology applications too. Electron tomography can be used to complement those studies where higher resolution is needed. In this article we present examples of utilizing these two 3D-EM techniques to study cellular organelles.

KW - 1182 Biochemistry, cell and molecular biology

M3 - Article

VL - 27

SP - 7

EP - 10

JO - Microscopy and Analysis

JF - Microscopy and Analysis

SN - 2049-4424

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ER -