Sammanfattning
We have developed a microchip based system to mimic metabolic reactions of
small organic compounds. The microchip, made of silicon, contains a titanium dioxide (TiO2) covered microreactor and an electrospray ionization (ESI) tip for direct mass spectrometric (MS) analysis. Phase I metabolic reactions were mimicked
using on-chip photocatalytic TiO2-layer and an external UV-light where the TiO2-
layer catalyzed chemical reactions. The reactions of test samples were quite similar
with biochemical reactions induced by hepatocytes or cytochrome P enzymes. The
microchip can be used for fast screening of phase I metabolic reactions and speed
up the drug discovery.
small organic compounds. The microchip, made of silicon, contains a titanium dioxide (TiO2) covered microreactor and an electrospray ionization (ESI) tip for direct mass spectrometric (MS) analysis. Phase I metabolic reactions were mimicked
using on-chip photocatalytic TiO2-layer and an external UV-light where the TiO2-
layer catalyzed chemical reactions. The reactions of test samples were quite similar
with biochemical reactions induced by hepatocytes or cytochrome P enzymes. The
microchip can be used for fast screening of phase I metabolic reactions and speed
up the drug discovery.
Originalspråk | engelska |
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Titel på värdpublikation | Proceedings of the 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2009), November 1-5, Jeju, Korea |
Redaktörer | Tae Song Kim, Yoon-Sik Lee |
Antal sidor | 3 |
Utgivningsort | Jeju |
Förlag | Chemical and Biological Microsystems Society |
Utgivningsdatum | 2009 |
Sidor | 1922-1924 |
Status | Publicerad - 2009 |
MoE-publikationstyp | A4 Artikel i en konferenspublikation |
Evenemang | International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2009) - Jeju, Sydkorea Varaktighet: 1 nov. 2009 → 5 nov. 2009 Konferensnummer: 13 |
Vetenskapsgrenar
- 116 Kemi