TiO2-Microchip combined to mass spectrometer for mimicking phase I metabolic reactions

Teemu Nissilä; Lauri Sainiemi; Mika-Matti Karikko; Marianna Leena Kemell; Mikko Ritala; Sami Franssila; Risto Kostiainen; Raimo Ketola

TiO2-Microchip combined to mass spectrometer for mimicking phase I metabolic reactions

Teemu Nissilä, Lauri Sainiemi, Mika-Matti Karikko, Marianna Leena Kemell, Mikko Ritala, Sami Franssila, Risto Kostiainen, Raimo Ketola

Forskningsoutput: Kapitel i bok/rapport/konferenshandling › Konferensbidrag › Vetenskaplig › Peer review

Sammanfattning

We have developed a microchip based system to mimic metabolic reactions of
small organic compounds. The microchip, made of silicon, contains a titanium dioxide (TiO2) covered microreactor and an electrospray ionization (ESI) tip for direct mass spectrometric (MS) analysis. Phase I metabolic reactions were mimicked
using on-chip photocatalytic TiO2-layer and an external UV-light where the TiO2-
layer catalyzed chemical reactions. The reactions of test samples were quite similar
with biochemical reactions induced by hepatocytes or cytochrome P enzymes. The
microchip can be used for fast screening of phase I metabolic reactions and speed
up the drug discovery.

Originalspråk	engelska
Titel på värdpublikation	Proceedings of the 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2009), November 1-5, Jeju, Korea
Redaktörer	Tae Song Kim, Yoon-Sik Lee
Antal sidor	3
Utgivningsort	Jeju
Förlag	Chemical and Biological Microsystems Society
Utgivningsdatum	2009
Sidor	1922-1924
Status	Publicerad - 2009
MoE-publikationstyp	A4 Artikel i en konferenspublikation
Evenemang	International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2009) - Jeju, Sydkorea Varaktighet: 1 nov. 2009 → 5 nov. 2009 Konferensnummer: 13

Vetenskapsgrenar

116 Kemi

Citera det här

Nissilä, T., Sainiemi, L., Karikko, M.-M., Kemell, M. L., Ritala, M., Franssila, S., Kostiainen, R., & Ketola, R. (2009). TiO2-Microchip combined to mass spectrometer for mimicking phase I metabolic reactions. I T. S. Kim, & Y.-S. Lee (Red.), Proceedings of the 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2009), November 1-5, Jeju, Korea (s. 1922-1924). Chemical and Biological Microsystems Society.

Nissilä, Teemu ; Sainiemi, Lauri ; Karikko, Mika-Matti et al. / TiO2-Microchip combined to mass spectrometer for mimicking phase I metabolic reactions. Proceedings of the 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2009), November 1-5, Jeju, Korea. redaktör / Tae Song Kim ; Yoon-Sik Lee. Jeju : Chemical and Biological Microsystems Society, 2009. s. 1922-1924

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title = "TiO2-Microchip combined to mass spectrometer for mimicking phase I metabolic reactions",

abstract = "We have developed a microchip based system to mimic metabolic reactions ofsmall organic compounds. The microchip, made of silicon, contains a titanium dioxide (TiO2) covered microreactor and an electrospray ionization (ESI) tip for direct mass spectrometric (MS) analysis. Phase I metabolic reactions were mimickedusing on-chip photocatalytic TiO2-layer and an external UV-light where the TiO2-layer catalyzed chemical reactions. The reactions of test samples were quite similarwith biochemical reactions induced by hepatocytes or cytochrome P enzymes. Themicrochip can be used for fast screening of phase I metabolic reactions and speedup the drug discovery.",

keywords = "116 Chemical sciences",

author = "Teemu Nissil{\"a} and Lauri Sainiemi and Mika-Matti Karikko and Kemell, {Marianna Leena} and Mikko Ritala and Sami Franssila and Risto Kostiainen and Raimo Ketola",

year = "2009",

language = "English",

pages = "1922--1924",

editor = "Kim, {Tae Song } and Yoon-Sik Lee",

booktitle = "Proceedings of the 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2009), November 1-5, Jeju, Korea",

publisher = "Chemical and Biological Microsystems Society",

address = "International",

note = "International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2009) ; Conference date: 01-11-2009 Through 05-11-2009",

}

Nissilä, T, Sainiemi, L, Karikko, M-M, Kemell, ML , Ritala, M, Franssila, S, Kostiainen, R & Ketola, R 2009, TiO2-Microchip combined to mass spectrometer for mimicking phase I metabolic reactions. i TS Kim & Y-S Lee (red), Proceedings of the 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2009), November 1-5, Jeju, Korea. Chemical and Biological Microsystems Society, Jeju, s. 1922-1924, International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2009), Jeju, Sydkorea, 01/11/2009.

TiO2-Microchip combined to mass spectrometer for mimicking phase I metabolic reactions. / Nissilä, Teemu; Sainiemi, Lauri; Karikko, Mika-Matti et al.
Proceedings of the 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2009), November 1-5, Jeju, Korea. red. / Tae Song Kim; Yoon-Sik Lee. Jeju: Chemical and Biological Microsystems Society, 2009. s. 1922-1924.

Forskningsoutput: Kapitel i bok/rapport/konferenshandling › Konferensbidrag › Vetenskaplig › Peer review

TY - GEN

T1 - TiO2-Microchip combined to mass spectrometer for mimicking phase I metabolic reactions

AU - Nissilä, Teemu

AU - Sainiemi, Lauri

AU - Karikko, Mika-Matti

AU - Kemell, Marianna Leena

AU - Ritala, Mikko

AU - Franssila, Sami

AU - Kostiainen, Risto

AU - Ketola, Raimo

N1 - Conference code: 13

PY - 2009

Y1 - 2009

N2 - We have developed a microchip based system to mimic metabolic reactions ofsmall organic compounds. The microchip, made of silicon, contains a titanium dioxide (TiO2) covered microreactor and an electrospray ionization (ESI) tip for direct mass spectrometric (MS) analysis. Phase I metabolic reactions were mimickedusing on-chip photocatalytic TiO2-layer and an external UV-light where the TiO2-layer catalyzed chemical reactions. The reactions of test samples were quite similarwith biochemical reactions induced by hepatocytes or cytochrome P enzymes. Themicrochip can be used for fast screening of phase I metabolic reactions and speedup the drug discovery.

AB - We have developed a microchip based system to mimic metabolic reactions ofsmall organic compounds. The microchip, made of silicon, contains a titanium dioxide (TiO2) covered microreactor and an electrospray ionization (ESI) tip for direct mass spectrometric (MS) analysis. Phase I metabolic reactions were mimickedusing on-chip photocatalytic TiO2-layer and an external UV-light where the TiO2-layer catalyzed chemical reactions. The reactions of test samples were quite similarwith biochemical reactions induced by hepatocytes or cytochrome P enzymes. Themicrochip can be used for fast screening of phase I metabolic reactions and speedup the drug discovery.

KW - 116 Chemical sciences

M3 - Conference contribution

SP - 1922

EP - 1924

BT - Proceedings of the 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2009), November 1-5, Jeju, Korea

A2 - Kim, Tae Song

A2 - Lee, Yoon-Sik

PB - Chemical and Biological Microsystems Society

CY - Jeju

T2 - International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2009)

Y2 - 1 November 2009 through 5 November 2009

ER -

Nissilä T, Sainiemi L, Karikko MM, Kemell ML , Ritala M, Franssila S et al. TiO2-Microchip combined to mass spectrometer for mimicking phase I metabolic reactions. I Kim TS, Lee YS, redaktörer, Proceedings of the 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (μTAS 2009), November 1-5, Jeju, Korea. Jeju: Chemical and Biological Microsystems Society. 2009. s. 1922-1924