Sammanfattning

Tree bark is a highly specialized array of tissues that plays important roles in plant protection and development. Bark tissues develop from two lateral meristems; the phellogen (cork cambium) produces the outermost stem-environment barrier called the periderm, while the vascular cambium contributes with phloem tissues. Although bark is diverse in terms of tissues, functions and species, it remains understudied at higher resolution. We dissected the stem of silver birch (Betula pendula) into eight major tissue types, and characterized these by a combined transcriptomics and metabolomics approach. We further analyzed the varying bark types within the Betulaceae family. The two meristems had a distinct contribution to the stem transcriptomic landscape. Furthermore, inter- and intraspecies analyses illustrated the unique molecular profile of the phellem. We identified multiple tissue-specific metabolic pathways, such as the mevalonate/betulin biosynthesis pathway, that displayed differential evolution within the Betulaceae. A detailed analysis of suberin and betulin biosynthesis pathways identified a set of underlying regulators and highlighted the important role of local, small-scale gene duplication events in the evolution of metabolic pathways. This work reveals the transcriptome and metabolic diversity among bark tissues and provides insights to its development and evolution, as well as its biotechnological applications.

Originalspråkengelska
TidskriftNew Phytologist
Volym222
Utgåva4
Sidor (från-till)1816-1831
Antal sidor16
ISSN0028-646X
DOI
StatusPublicerad - jun 2019
MoE-publikationstypA1 Tidskriftsartikel-refererad

Vetenskapsgrenar

  • 1182 Biokemi, cell- och molekylärbiologi

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@article{716b7759a7834c3db95ab688f68535cd,
title = "Tissue-specific study across the stem reveals the chemistry and transcriptome dynamics of birch bark.",
abstract = "Tree bark is a highly specialized array of tissues that plays important roles in plant protection and development. Bark tissues develop from two lateral meristems; the phellogen (cork cambium) produces the outermost stem-environment barrier called the periderm, while the vascular cambium contributes with phloem tissues. Although bark is diverse in terms of tissues, functions and species, it remains understudied at higher resolution. We dissected the stem of silver birch (Betula pendula) into eight major tissue types, and characterized these by a combined transcriptomics and metabolomics approach. We further analyzed the varying bark types within the Betulaceae family. The two meristems had a distinct contribution to the stem transcriptomic landscape. Furthermore, inter- and intraspecies analyses illustrated the unique molecular profile of the phellem. We identified multiple tissue-specific metabolic pathways, such as the mevalonate/betulin biosynthesis pathway, that displayed differential evolution within the Betulaceae. A detailed analysis of suberin and betulin biosynthesis pathways identified a set of underlying regulators and highlighted the important role of local, small-scale gene duplication events in the evolution of metabolic pathways. This work reveals the transcriptome and metabolic diversity among bark tissues and provides insights to its development and evolution, as well as its biotechnological applications.",
keywords = "1182 Biochemistry, cell and molecular biology, bark, Betula pendula (silver birch), cambium, genome evolution, metabolic pathways, periderm, phellem, phellogen, BETULINIC ACID, SUBERIN BIOSYNTHESIS, WOOD FORMATION, CELL-WALLS, GENOME, GENE, ARABIDOPSIS, TRITERPENOIDS, PROFILES, PHOTOSYNTHESIS",
author = "{Alonso Serra}, {Juan Antonio} and Omid Safronov and Kean-Jin Lim and Sara Fraser-Miller and Blokhina, {Olga Borisovna} and Ana Campilho and Sun-Li Chong and Fagerstedt, {Kurt Valter} and Haavikko, {Raisa Hannele} and Yk{\"a} Helariutta and Immanen, {Juha Jouko Matias} and Kangasj{\"a}rvi, {Jaakko Sakari} and Kauppila, {Tiina J.} and Mari Lehtonen and Laura Ragni and Sitaram Rajaraman and Riikka-Marjaana R{\"a}s{\"a}nen and Pezhman Safdari and Tenkanen, {Tiina Maija} and Yli-Kauhaluoma, {Jari Tapani} and Teeri, {Teemu Heikki} and Strachan, {Clare Joanna} and Kaisa Nieminen and Saloj{\"a}rvi, {Jarkko Tapani}",
year = "2019",
month = "6",
doi = "10.1111/nph.15725",
language = "English",
volume = "222",
pages = "1816--1831",
journal = "New Phytologist",
issn = "0028-646X",
publisher = "Wiley",
number = "4",

}

TY - JOUR

T1 - Tissue-specific study across the stem reveals the chemistry and transcriptome dynamics of birch bark.

AU - Alonso Serra, Juan Antonio

AU - Safronov, Omid

AU - Lim, Kean-Jin

AU - Fraser-Miller, Sara

AU - Blokhina, Olga Borisovna

AU - Campilho, Ana

AU - Chong, Sun-Li

AU - Fagerstedt, Kurt Valter

AU - Haavikko, Raisa Hannele

AU - Helariutta, Ykä

AU - Immanen, Juha Jouko Matias

AU - Kangasjärvi, Jaakko Sakari

AU - Kauppila, Tiina J.

AU - Lehtonen, Mari

AU - Ragni, Laura

AU - Rajaraman, Sitaram

AU - Räsänen, Riikka-Marjaana

AU - Safdari, Pezhman

AU - Tenkanen, Tiina Maija

AU - Yli-Kauhaluoma, Jari Tapani

AU - Teeri, Teemu Heikki

AU - Strachan, Clare Joanna

AU - Nieminen, Kaisa

AU - Salojärvi, Jarkko Tapani

PY - 2019/6

Y1 - 2019/6

N2 - Tree bark is a highly specialized array of tissues that plays important roles in plant protection and development. Bark tissues develop from two lateral meristems; the phellogen (cork cambium) produces the outermost stem-environment barrier called the periderm, while the vascular cambium contributes with phloem tissues. Although bark is diverse in terms of tissues, functions and species, it remains understudied at higher resolution. We dissected the stem of silver birch (Betula pendula) into eight major tissue types, and characterized these by a combined transcriptomics and metabolomics approach. We further analyzed the varying bark types within the Betulaceae family. The two meristems had a distinct contribution to the stem transcriptomic landscape. Furthermore, inter- and intraspecies analyses illustrated the unique molecular profile of the phellem. We identified multiple tissue-specific metabolic pathways, such as the mevalonate/betulin biosynthesis pathway, that displayed differential evolution within the Betulaceae. A detailed analysis of suberin and betulin biosynthesis pathways identified a set of underlying regulators and highlighted the important role of local, small-scale gene duplication events in the evolution of metabolic pathways. This work reveals the transcriptome and metabolic diversity among bark tissues and provides insights to its development and evolution, as well as its biotechnological applications.

AB - Tree bark is a highly specialized array of tissues that plays important roles in plant protection and development. Bark tissues develop from two lateral meristems; the phellogen (cork cambium) produces the outermost stem-environment barrier called the periderm, while the vascular cambium contributes with phloem tissues. Although bark is diverse in terms of tissues, functions and species, it remains understudied at higher resolution. We dissected the stem of silver birch (Betula pendula) into eight major tissue types, and characterized these by a combined transcriptomics and metabolomics approach. We further analyzed the varying bark types within the Betulaceae family. The two meristems had a distinct contribution to the stem transcriptomic landscape. Furthermore, inter- and intraspecies analyses illustrated the unique molecular profile of the phellem. We identified multiple tissue-specific metabolic pathways, such as the mevalonate/betulin biosynthesis pathway, that displayed differential evolution within the Betulaceae. A detailed analysis of suberin and betulin biosynthesis pathways identified a set of underlying regulators and highlighted the important role of local, small-scale gene duplication events in the evolution of metabolic pathways. This work reveals the transcriptome and metabolic diversity among bark tissues and provides insights to its development and evolution, as well as its biotechnological applications.

KW - 1182 Biochemistry, cell and molecular biology

KW - bark

KW - Betula pendula (silver birch)

KW - cambium

KW - genome evolution

KW - metabolic pathways

KW - periderm

KW - phellem

KW - phellogen

KW - BETULINIC ACID

KW - SUBERIN BIOSYNTHESIS

KW - WOOD FORMATION

KW - CELL-WALLS

KW - GENOME

KW - GENE

KW - ARABIDOPSIS

KW - TRITERPENOIDS

KW - PROFILES

KW - PHOTOSYNTHESIS

U2 - 10.1111/nph.15725

DO - 10.1111/nph.15725

M3 - Article

VL - 222

SP - 1816

EP - 1831

JO - New Phytologist

JF - New Phytologist

SN - 0028-646X

IS - 4

ER -