Sammanfattning
Tree bark is a highly specialized array of tissues that plays important roles in plant protection and development. Bark tissues develop from two lateral meristems; the phellogen (cork cambium) produces the outermost stem-environment barrier called the periderm, while the vascular cambium contributes with phloem tissues. Although bark is diverse in terms of tissues, functions and species, it remains understudied at higher resolution. We dissected the stem of silver birch (Betula pendula) into eight major tissue types, and characterized these by a combined transcriptomics and metabolomics approach. We further analyzed the varying bark types within the Betulaceae family. The two meristems had a distinct contribution to the stem transcriptomic landscape. Furthermore, inter- and intraspecies analyses illustrated the unique molecular profile of the phellem. We identified multiple tissue-specific metabolic pathways, such as the mevalonate/betulin biosynthesis pathway, that displayed differential evolution within the Betulaceae. A detailed analysis of suberin and betulin biosynthesis pathways identified a set of underlying regulators and highlighted the important role of local, small-scale gene duplication events in the evolution of metabolic pathways. This work reveals the transcriptome and metabolic diversity among bark tissues and provides insights to its development and evolution, as well as its biotechnological applications.
| Originalspråk | engelska |
|---|---|
| Tidskrift | New Phytologist |
| Volym | 222 |
| Utgåva | 4 |
| Sidor (från-till) | 1816-1831 |
| Antal sidor | 16 |
| ISSN | 0028-646X |
| DOI | |
| Status | Publicerad - jun 2019 |
| MoE-publikationstyp | A1 Tidskriftsartikel-refererad |
Vetenskapsgrenar
- 1182 Biokemi, cell- och molekylärbiologi
Citera det här
}
Tissue-specific study across the stem reveals the chemistry and transcriptome dynamics of birch bark. / Alonso Serra, Juan Antonio; Safronov, Omid; Lim, Kean-Jin; Fraser-Miller, Sara ; Blokhina, Olga Borisovna; Campilho, Ana; Chong, Sun-Li; Fagerstedt, Kurt Valter; Haavikko, Raisa Hannele; Helariutta, Ykä; Immanen, Juha Jouko Matias; Kangasjärvi, Jaakko Sakari; Kauppila, Tiina J.; Lehtonen, Mari ; Ragni, Laura; Rajaraman, Sitaram; Räsänen, Riikka-Marjaana; Safdari, Pezhman; Tenkanen, Tiina Maija; Yli-Kauhaluoma, Jari Tapani; Teeri, Teemu Heikki; Strachan, Clare Joanna; Nieminen, Kaisa; Salojärvi, Jarkko Tapani.
I: New Phytologist, Vol. 222, Nr. 4, 06.2019, s. 1816-1831.Forskningsoutput: Tidskriftsbidrag › Artikel › Vetenskaplig › Peer review
TY - JOUR
T1 - Tissue-specific study across the stem reveals the chemistry and transcriptome dynamics of birch bark.
AU - Alonso Serra, Juan Antonio
AU - Safronov, Omid
AU - Lim, Kean-Jin
AU - Fraser-Miller, Sara
AU - Blokhina, Olga Borisovna
AU - Campilho, Ana
AU - Chong, Sun-Li
AU - Fagerstedt, Kurt Valter
AU - Haavikko, Raisa Hannele
AU - Helariutta, Ykä
AU - Immanen, Juha Jouko Matias
AU - Kangasjärvi, Jaakko Sakari
AU - Kauppila, Tiina J.
AU - Lehtonen, Mari
AU - Ragni, Laura
AU - Rajaraman, Sitaram
AU - Räsänen, Riikka-Marjaana
AU - Safdari, Pezhman
AU - Tenkanen, Tiina Maija
AU - Yli-Kauhaluoma, Jari Tapani
AU - Teeri, Teemu Heikki
AU - Strachan, Clare Joanna
AU - Nieminen, Kaisa
AU - Salojärvi, Jarkko Tapani
PY - 2019/6
Y1 - 2019/6
N2 - Tree bark is a highly specialized array of tissues that plays important roles in plant protection and development. Bark tissues develop from two lateral meristems; the phellogen (cork cambium) produces the outermost stem-environment barrier called the periderm, while the vascular cambium contributes with phloem tissues. Although bark is diverse in terms of tissues, functions and species, it remains understudied at higher resolution. We dissected the stem of silver birch (Betula pendula) into eight major tissue types, and characterized these by a combined transcriptomics and metabolomics approach. We further analyzed the varying bark types within the Betulaceae family. The two meristems had a distinct contribution to the stem transcriptomic landscape. Furthermore, inter- and intraspecies analyses illustrated the unique molecular profile of the phellem. We identified multiple tissue-specific metabolic pathways, such as the mevalonate/betulin biosynthesis pathway, that displayed differential evolution within the Betulaceae. A detailed analysis of suberin and betulin biosynthesis pathways identified a set of underlying regulators and highlighted the important role of local, small-scale gene duplication events in the evolution of metabolic pathways. This work reveals the transcriptome and metabolic diversity among bark tissues and provides insights to its development and evolution, as well as its biotechnological applications.
AB - Tree bark is a highly specialized array of tissues that plays important roles in plant protection and development. Bark tissues develop from two lateral meristems; the phellogen (cork cambium) produces the outermost stem-environment barrier called the periderm, while the vascular cambium contributes with phloem tissues. Although bark is diverse in terms of tissues, functions and species, it remains understudied at higher resolution. We dissected the stem of silver birch (Betula pendula) into eight major tissue types, and characterized these by a combined transcriptomics and metabolomics approach. We further analyzed the varying bark types within the Betulaceae family. The two meristems had a distinct contribution to the stem transcriptomic landscape. Furthermore, inter- and intraspecies analyses illustrated the unique molecular profile of the phellem. We identified multiple tissue-specific metabolic pathways, such as the mevalonate/betulin biosynthesis pathway, that displayed differential evolution within the Betulaceae. A detailed analysis of suberin and betulin biosynthesis pathways identified a set of underlying regulators and highlighted the important role of local, small-scale gene duplication events in the evolution of metabolic pathways. This work reveals the transcriptome and metabolic diversity among bark tissues and provides insights to its development and evolution, as well as its biotechnological applications.
KW - 1182 Biochemistry, cell and molecular biology
KW - bark
KW - Betula pendula (silver birch)
KW - cambium
KW - genome evolution
KW - metabolic pathways
KW - periderm
KW - phellem
KW - phellogen
KW - BETULINIC ACID
KW - SUBERIN BIOSYNTHESIS
KW - WOOD FORMATION
KW - CELL-WALLS
KW - GENOME
KW - GENE
KW - ARABIDOPSIS
KW - TRITERPENOIDS
KW - PROFILES
KW - PHOTOSYNTHESIS
U2 - 10.1111/nph.15725
DO - 10.1111/nph.15725
M3 - Article
VL - 222
SP - 1816
EP - 1831
JO - New Phytologist
JF - New Phytologist
SN - 0028-646X
IS - 4
ER -