Ultrasound-Enhanced Fine-Needle Biopsy Improves Yield in Human Epithelial and Lymphoid Tissue

Yohann Le Bourlout, Minna Rehell, Jetta Kelppe, Jaana Rautava, Emanuele Perra, Jouni Rantanen, Gösta Ehnholm, Nick Hayward, Kristofer Nyman, Kenneth P.H. Pritzker, Jussi Tarkkanen, Timo Atula, Katri Aro, Heikki J. Nieminen

Forskningsoutput: TidskriftsbidragArtikelVetenskapligPeer review

Sammanfattning

Objective: Needle biopsy is a common technique used to obtain cell and tissue samples for diagnostics. Currently, two biopsy methods are widely used: (i) fine-needle aspiration biopsy (FNAB) and (ii) core needle biopsy (CNB). However, these methods have limitations. Recently, we developed ultrasound-enhanced fine-needle aspiration biopsy (USeFNAB), which employs a needle that flexurally oscillates at an ultrasonic frequency of ∼32 kHz. The needle motion contributes to increased tissue collection while preserving cells and tissue constructs for pathological assessment. Previously, USeFNAB has been investigated only in ex vivo animal tissue. The present study was aimed at determining the feasibility of using USeFNAB in human epithelial and lymphoid tissue. Methods: Needle biopsy samples were acquired using FNAB, CNB and USeFNAB on ex vivo human tonsils (N = 10). The tissue yield and quality were quantified by weight measurement and blinded pathologists’ assessments. The biopsy methods were then compared. Results: The results revealed sample mass increases of, on average, 2.3- and 5.4-fold with USeFNAB compared with the state-of-the-art FNAB and CNB, respectively. The quality of tissue fragments collected by USeFNAB was equivalent to that collected by the state-of-the-art methods in terms of morphology and immunohistochemical stainings made from cell blocks as judged by pathologists. Conclusion: Our study indicates that USeFNAB is a promising method that could improve tissue yield to ensure sufficient material for ancillary histochemical and molecular studies for diagnostic pathology, thereby potentially increasing diagnostic accuracy.

Originalspråkengelska
TidskriftUltrasound in Medicine and Biology
Volym50
Nummer8
Sidor (från-till)1247-1254
Antal sidor8
ISSN0301-5629
DOI
StatusPublicerad - 2024
MoE-publikationstypA1 Tidskriftsartikel-refererad

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  • 217 Medicinsk teknik
  • 3121 Allmänmedicin, inre medicin och annan klinisk medicin

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