Quantification of cell death and proliferation of patient-derived ovarian cancer organoids through 3D imaging and image analysis

Aikaterini Skorda; Anna Røssberg Lauridsen; Kaisa Huhtinen; Alexandra Lahtinen; Wojciech Senkowski; Jaana Oikkonen; Johanna Hynninen; Sampsa Hautaniemi; Tuula Kallunki

doi:10.1016/j.xpro.2023.102683

Quantification of cell death and proliferation of patient-derived ovarian cancer organoids through 3D imaging and image analysis

Aikaterini Skorda
, Anna Røssberg Lauridsen
, Kaisa Huhtinen
, Alexandra Lahtinen
, Wojciech Senkowski
, Jaana Oikkonen
, Johanna Hynninen
, Sampsa Hautaniemi
, Tuula Kallunki

Tutkimustuotos: Artikkelijulkaisu › Artikkeli › Tieteellinen › vertaisarvioitu

Alkuperäiskieli	englanti
Artikkeli	102683
Lehti	STAR protocols
Vuosikerta	4
Numero	4
Sivumäärä	17
ISSN	2666-1667
DOI - pysyväislinkit	https://doi.org/10.1016/j.xpro.2023.102683
Tila	Julkaistu - 15 jouluk. 2023
OKM-julkaisutyyppi	A1 Alkuperäisartikkeli tieteellisessä aikakauslehdessä, vertaisarvioitu

Lisätietoja

Funding Information:
This project received funding from the European Union’s Horizon 2020 Research and Innovation Program under grant agreement no. 965193 (DECIDER). We are grateful to Krister Wennerberg for advice, good discussions, and sharing HGSC organoid culture protocols prior to their publication. We acknowledge the Bioimaging Core Facility for the access and assistance in regards to imaging. We acknowledge Tiina Naumanen Dietrich for expert assistance in image acquisition with ImageXpress HT.ai and image analysis with the MetaXpress software, Ahmad Abdul-Al for careful assistance in maintaining and culturing of PDOs, and Peppi Alho for skillful assistance with fresh tumor processing.

Publisher Copyright:
© 2023 The Author(s)

Tieteenalat

3122 Syöpätaudit
1182 Biokemia, solu- ja molekyylibiologia

Pääsy asiakirjaan

10.1016/j.xpro.2023.102683

Quantification of cell death and proliferation of patient-derived ovarian cancer organoids through 3D imaging and image analysisLopullinen julkaistu versio, 4,69 MBLisenssi: CC BY

Siteeraa tätä

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author = "Aikaterini Skorda and Lauridsen, \{Anna R{\o}ssberg\} and Kaisa Huhtinen and Alexandra Lahtinen and Wojciech Senkowski and Jaana Oikkonen and Johanna Hynninen and Sampsa Hautaniemi and Tuula Kallunki",

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T1 - Quantification of cell death and proliferation of patient-derived ovarian cancer organoids through 3D imaging and image analysis

AU - Skorda, Aikaterini

AU - Lauridsen, Anna Røssberg

AU - Huhtinen, Kaisa

AU - Lahtinen, Alexandra

AU - Senkowski, Wojciech

AU - Oikkonen, Jaana

AU - Hynninen, Johanna

AU - Hautaniemi, Sampsa

AU - Kallunki, Tuula

PY - 2023/12/15

Y1 - 2023/12/15

KW - High-Throughput Screening

KW - Organoids

KW - Cancer

KW - Cell Biology

KW - 3122 Cancers

KW - 1182 Biochemistry, cell and molecular biology

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DO - 10.1016/j.xpro.2023.102683

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C2 - 37976153

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SN - 2666-1667

VL - 4

JO - STAR protocols

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